Application Note: Homogeneous Add-Mix-Read Assay for Total and Oxidized Glutathione
28 August 2015

A wide variety of compounds can induce intracellular formation of reactive oxygen species (ROS) and the resulting cellular damage can eventually lead to apoptosis or necrosis. Measuring the ratio of reduced to oxidized glutathione (GSH/GSSG ratio) is a way to determine how such compounds alter the redox potential in the cell. GSH/GSSG-Glo allows for rapid, direct measurement of total and oxidized glutathione in cell-culture wells using low cell density.