Fully Integrated Plating and Colony Picking for Synthetic Biology Workflows

The Institute for Research in Immunology and Cancer (IRIC) at the University of Montreal pursues a unique approach to investigate complex biological processes. In this study, the laboratory team established a reliable, fully automated cloning workflow on a Freedom EVO^® workstation. This system performs fully automated multi-fragment DNA assembly, customized plating in ANSI/SLAS-format plates, colony picking and PCR set-up – as well as agar plate preparation – allowing the group to greatly increase the throughput of their experiments.