Application Note: Fluorescence Polarization Based Assay Suitable for Screening for H-Prostaglandin D Synthase Inhibitors
13 August 2015

Prostaglandins are a group of lipid compounds that are involved in diverse effects in animal physiology. Prostaglandin D2 (PGD2) has been characterized for its role in asthma where its concentration has been shown to be 10 times higher in asthma patients leading to bronchial airway contraction after exposure to an allergen. For this and other reasons, inhibitors are sought for the hematopoietic prostaglandin D synthase (H-PGDS) which catalyzes the final PGD2 biosynthesis step. This application note evaluates the performance of Cayman Chemicals green FP-based inhibitor assay screening kit. The kit was tested with both the CLARIOstar and PHERAstar FS. Settings more suitable for high throughput screening (HTS) were also tested.