Application Note: Determination of Drug Residence Time in an HTS Format using Kinetic Analysis with the Transcreener® ADP2 Assay
20 June 2016
This poster describes a systematic approach to determine the residence time of compounds using Abl1 and Aurora C as targets and several well characterized ATP-competitive drugs. Residence time can be determined by equilibrium binding measurements using methods such as surface plasmon resonance (SPR), which provides highly quantitative data but requires costly instrumentation and long experimental timelines. Transcreener® ADP2 Assays offer a highly sensitive and versatile method for estimating drug residence times for kinases using a multimode fluorescent plate reader.