Application Note: Cell-Free Protein Synthesis with 2H/15N/13C-Labeled Amino Acids in H2O for the Production of Perdeuterated Proteins with 1H in the Exchangeable Positions
25 June 2017

This study illustrates the finding that backexchange is redundant if the protein of interest is produced by cellfree synthesis from perdeuterated amino acids in H2O. Importantly, the costs associated with cellfree synthesis compare favorably with those of in vivo expression.