Application Note: Analysis of Ethylglucuronide/Ethylsulfate in the Urine of Sexual Assault Cases
9 December 2014

In this study, samples of urine taken from completed and signed out cases (ethanol related sexual assault) were analyzed for the concentrations of ethylglucuronide (EtG) and ethylsulfate (EtS) using solid phase extraction and LC-MS/MS. This study was initiated to develop a simple, robust method that would assist forensic analysts in the quantification of EtG/EtS in urine samples. The developed method was then applied to genuine (ethanol) cases to determine the concentrations of both EtG/EtS where the ethanol in the urine sample was negative, but had been suspected.