Application Note: A Simplified Solid Phase Extraction (SPE) Protocol for Bioanalysis Using Oasis HLB
7 October 2014
This application note describes a simple, 3-step SPE protocol using OasisHLB in µElution Plates and 1 cc cartridges. This protocol saves an average 40% processing time, 70% solvent consumption, and provides a simple method that works for a wide range of acidic, neutral, and basic analytes. This simplified, 3-step protocol consists of load, wash, and elution, thus eliminating the conditioning and equilibration steps.