A New Lipid Software Workflow for Processing Orbitrap-based Global Lipidomics Data in Translational and Systems Biology Research

Thermo Scientific Accela - HPLC and U-HPLC in a Single System The Thermo Accela™ high-speed chromatographic system provides fast, efficient chromatographic separations over an expansive range of flow rates and pressures. Thermo Accela is designed to optimize performance of sub-two micron particle columns, providing seamless operation spanning conventional LC pressures from short LC columns, up to 15,000 psi for long-column separations of complex bio mixtures. Features of the Thermo Accela include: The Accela Pump assures rapid and reproducible transfer of even the most complex and aggressive gradients. This quaternary pump is capable of handling pressures up to 15,000 psi with a delay volume of only 65 µL, enabling high speed chromatographic separations. The Accela Autosampler has a specialized diamond coated high pressure valve that can handle the rigors of constant high pressure injections while the unique sampling design enables 30 second injection cycles. This autosampler integrates isothermal injection and separation to provide superior reproducibility by eliminating all external environmental influences to the chromatography. The Accela PDA has been optimized for the detection of high speed chromatographic separations. The short 1 cm flow cell pathlength combined with the incredible minimized flow cell volume of 2 µL, renders this Patented LightPipe™ flowcell as the premium choice for fast separations. The extremely low level of dispersion in the LightPipe flowcell retains the excellent peak shape and chromatographic resolution from the column. Thermo Scientific Accela, coupled with the sub-two micron particle columns, provides fast, controlled separations with high efficiency and resolution, accelerating LC and LC/MS applications. The High Speed LC System for today’s modern laboratory. Advantages of the Thermo Accela include:  Optimized system delay volumes for fast separations Flexible sample format accepts vials or plates Quaternary pump capable of conventional and ultra-high pressures Patented LightPipe technology provides maximum sensitivity and resolution Complete chromatography solution with Thermo mass spectrometers

The Exactive Plus Orbitrap LC-MS is a benchtop full scan mass spectrometer designed for compound identification and high-throughput, high-performance screening. The Exactive Plus delivers high resolution accurate mass (HRAM) data and fast, reproducible results for qualitative and quantitative analysis. The availability of HRAM and full scan capability gives you all the data, all the time. It allows retrospective data analysis and eliminates MS method development leading to improved and simplified analytical throughput.Exactive Plus is easy-to-use, cost effective to operate and coupled with workflow-driven software, makes an ideal instrument for new users in routine analytical laboratories.Selected benefits of the Exactive Plus are specified below: High Resolving Power (up to 140,000) - Eliminates isobaric interferences to increase confidence for analysis or complex samples Mass accuracy - Better than 1ppm in full scan and AIF scan using fully automated AGC and mass calibration procedures offers confident identification U-HPLC Scan Speed - The 12Hz scanning frequency of Exactive Plus is fully compatible with U-HPLC and ensures exact mass measurement for fast chromatography applications Fast Polarity Switching - A full cycle of 1 positive and 1 negative scan is performed within 1 second at a resolution setting of 35,000 without sacrificing mass accuracy All-Ion-Fragmentation (AIF) - With multiple dissociation techniques; in source collisional induced dissociation (CID) and high energy collisional dissociation (HCD), provides MS and MS/MS data Full MS scan, AIF scan, data dependent AIF, alternating Full MS and AIF scan Intuitive software interface The Exactive Plus Orbitrap LC-MS is ideal for the following applications: Environmental and Food Safety Clinical Research Forensic Toxicology Drug discovery, metabolism and pharmacokinetics (DMPK) Metabolomics Pharmaceutical BioPharma – Intact Protein analysis Laboratories analyzing food, pharmaceuticals, neutraceuticals, and other complex samples can now purchase Exactive Plus LC-MS system equipped from the factory with IonSense ID-CUBE™ ion sources. This configuration is designed to minimize the need for sample preparation to simplify and speed analyses.