Application Note: SuperNova v428: New bright polymer dye for flow cytometry applications
22 April 2021
SuperNova v428 is a new violet laser (405 nm)-excitable water-soluble polymer dye which shows exceptional brightness. This is attributed to its high extinction coefficient (~3 million Lmol-1 cm-1) and fluorescence quantum yield (~0.6) in phosphate buffer. Excitation maximum of this polymer is measured as 414 nm and its emission maximum is at 428 nm (Figure 1) which can be detected using a 450/50 bandpass filter or equivalent in a flow cytometer.