Application Note: Simultaneous, high-yield extraction of DNA and RNA from FFPE tissue
30 April 2021
Simultaneous extraction and purification of both RNA and DNA from FFPE tissue samples is critical to enable multiple downstream analyses from rare or limited samples. To avoid yield loss, most technologies use a serial isolation approach where DNA and RNA are separated after lysis by filtration or precipitation before entering separate purification workflows. This results in twice the number of purification steps per sample. With simultaneous extraction and purification of RNA and DNA from FFPE tissue samples without yield loss, the Ionic® FFPE Complete Purification Kit for the Ionic® Purification System offers a significant savings on total workflow time and reduces hands-on time by more than 75%.