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Quantikine ELISAs and proteome profiler antibody arrays enable COVID-19 biomarker research

Bio-Techne
27 Nov 2020

In this publication spotlight, R&D systems, a Bio-Techne brand, outlines how its Proteome Profiler Antibody Arrays & Quantikine ELISAs may be used to investigate the relationship between novel biomarker candidates, the cytokine storm and COVID-19 severity.

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Human VEGF Quantikine ELISA Kit

Bio-Techne

Human VEGF Quantikine ELISA Kit - Human VEGF Immunoassay for the quantitative determination of human vascular endothelial growth factor (VEGF) concentrations in cell culture supernates, serum, and plasma. The R&D Quantikine Human VEGF ELISA is a 4.5 hour solid phase ELISA. This R&D ELISA employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for VEGF has been pre-coated onto a microplate. R&D Systems Quantikine ELISA Kits are complete and ready-to-use. Features of the R&D Quantikine ELISA Kit include: • Complete ready to use kits • Exhaustively tested for superior quality and reproducibility • Detailed protocol booklets include inter- and intra-assay precision, recovery, linearity, sensitivity, and sample value data • Colorimetric detection R&D Quantikine ELISA Kit Contents:Kit contents may vary slightly. • Pre-coated 96-well Microplate • Conjugated Detection Antibody • Calibrated Immunoassay Standard • Assay Diluent • Calibrator Diluent(s) • Wash Buffer • Color Reagent A and B • Stop Solution • Plate Sealers

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Proteome Profiler Antibody Arrays

Bio-Techne

R&D Proteome Profiler Antibody Arrays R&D Proteome Profiler Arrays allow for the measurement of multiple proteins in a single sample. These arrays require no specialized equipment, and eliminate the need for multiple immunoprecipitation/Western blot experiments. R&D Array kits contain buffers, detection antibodies, and membranes spotted in duplicate with capture antibodies carefully selected for their specificity. R&D Arrays include:R&D Systems Human Phospho-RTK Antibody Array (Catalog # ARY001)Detect the relative site-specific phosphorylation levels of 42 different receptor tyrosine kinases(RTKs). R&D Systems Human Phospho-MAPK Antibody Array (Catalog # ARY002) Detect the relative site-specific phosphorylation levels of 19 different intracellular serine/threonine kinases. R&D Systems Human Phospho-Kinase Antibody Array (Catalog # ARY003) Detect the relative phosphorylation levels of 46 intracellular serine/threonine/tyrosine kinases. R&D Systems Human Phospho-Immunoreceptor Antibody Array (Catalog # ARY004) Detect the relative phosphorylation levels of 59 ITAM/ITIM-associated immunoreceptors. R&D Systems Human Cytokine Antibody Array, Panel A (Catalog # ARY005) Detect the relative levels of 36 cytokines, chemokines, and acute phase proteins simultaneously. R&D Systems Mouse Cytokine Antibody Array, Panel A (Catalog # ARY006) Detect the relative levels of 40 cytokines, chemokines, and more simultaneously. R&D Systems Human Angiogenesis Antibody Array (Catalog # ARY007) Detect the relative levels of 55 angiogenesis-related proteins simultaneously. R&D Systems Rat Cytokine Antibody Array (Catalog # ARY008) Detect the relative levels of 29 cytokines, chemokines and more simultaneously. R&D Systems Human Apoptosis Antibody Array (Catalog # ARY009) Detect the relative levels of 35 apoptosis-related proteins simultaneously. R&D Systems Human Pluripotent Stem Cell Antibody Array (Catalog # ARY010) Detect the relative levels of 15 pluripotent stem cell markers simultaneously.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.CytokinesCytokines are signaling proteins critical for cell communication in the immune system. They regulate inflammation, immune responses, and cell growth. Cytokine research is vital for understanding disease mechanisms and developing therapies. Explore tools for cytokine detection and analysis in our directory.Respiratory InfectionsRespiratory infections are caused by pathogens such as bacteria, viruses, and fungi, leading to diseases like pneumonia, tuberculosis, and COVID-19. It is important to identify respiratory infections quickly in patients to ensure appropriate treatment and care, and to prevent sepsis. Browse our peer-reviewed product directory to find the best tools for respiratory infection detection, compare products, check reviews, and get pricing directly from manufacturers.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.Cell Based AssaysCell-based assays use living cells to measure biological responses to various treatments or stimuli. They are essential for drug discovery, toxicology testing, and understanding cellular mechanisms. These assays are used to assess cell viability, proliferation, gene expression, and other cellular activities. Browse our peer-reviewed product directory to find the best cell-based assay tools and reagents, compare products, read customer reviews, and get pricing directly from manufacturers.CoronavirusCoronaviruses are a large family of viruses that can cause illnesses ranging from the common cold to more severe diseases like COVID-19. Research into the biology, transmission, and treatment of coronaviruses has become a global priority, particularly since the emergence of SARS-CoV-2. Understanding the virus�s structure and behavior is essential for developing vaccines, diagnostics, and antiviral treatments. Explore our peer-reviewed product directory to discover the latest research tools, diagnostic tests, vaccines, and treatments for coronavirus, compare products, read reviews, and receive pricing directly from manufacturers.Covid-19COVID-19, caused by the SARS-CoV-2 virus, has led to a global health crisis with profound impacts on societies and economies. Research into diagnostics, vaccines, treatments, and understanding viral mechanisms continues to evolve. The pandemic has accelerated innovations in healthcare and biopharmaceuticals, including novel vaccine platforms and rapid testing technologies. Browse our peer-reviewed product directory to find top COVID-19 testing kits, research tools, vaccines, and treatments, compare products, read reviews, and receive direct pricing from manufacturers.Infectious DiseasesInfectious diseases are caused by pathogens such as bacteria, viruses, and fungi. Effective detection, prevention, and treatment are crucial to control outbreaks and improve public health. Diagnostic tools, vaccines, and antimicrobial treatments are essential components of managing these diseases. Research into emerging pathogens and resistance patterns continues to evolve. Browse our peer-reviewed product directory to find the best diagnostic tools, prevention solutions, and treatment options; compare products, read customer reviews, and get pricing directly from manufacturers.

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