Application Note: Long-Term Hepatotoxicity Studies Using Cultured Human iPSC Derived Hepatocytes
9 November 2018
Quantitative microscopy was employed to determine the effect of drugs on human iPSC derived hepatocytes aggregated into spheroids. Three phenotypes of toxicity were measured using fluorescent probes: reactive oxygen species generation, mitochondrial membrane potential decline and plasma membrane rupture. Results were compared to hepatocytes cultured in flat bottom microplates. Spheroid formation enabled much longer kinetic studies, extending out to 14 days. This capability allowed for measurement of full pharmacology and phenotype.