Application Note: HILIC analysis of oligonucleotides using bioinert columns
15 February 2023

As oligonucleotides have a wide range of applications, they have the potential to treat or manage a variety of diseases. Therefore, robust and sensitive analytical methods are required. In this application note, three different oligonucleotide mixtures (dT15-35 and dT40-100 deoxythymidine oligonucleotides and rU15-30 RNA oligonucleotides) were analyzed using a YMC-Triart Diol HILIC column with conventional stainless steel hardware and the bioinert column option YMC-Accura Triart Diol-HILIC column. The comparison demonstrates that using a bioinert coated column results in higher peak areas, higher intensities and less tailing.