FDA Validated Molecular Method to Detect C. cayetanensis in Food Samples
16 May 2019Download this method to learn how the FastPrep-24™ homogenizer can be used used in combination with the FastDNA™ Spin Kit for Soil and is shown to be an effective method for the lysis of C. cayetanensis oocysts from infected food matrices and isolation of their DNA. The extracted DNA was used successfully in real-time PCR assays that were able to detect as few as 5 oocysts in 25 g of food samples.
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FastDNA SPIN Kit for Soil™
MP BiomedicalsIsolate Pure DNA from Cells Present in Soil or Other Environmental Samples! Thorough lysis in seconds of any microorganism present in environmental samples. Ready-to-use DNA for quantitative and qualitative characterization of microbial soil communities. Excellent reproducibility for an optimum assay-to-assay consistency. Total removal of humic acids and PCR inhibitors for a successful investigation of microbial diversity. The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungal, plant and animal genomic DNA from soil and environmental samples. Up to 500 mg soil are processed by the FastPrep®-24 5G or and any bead beating homogenizers with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter method and is suitable for PCR and other downstream applications.
FastPrep-24™ Classic Instrument
MP BiomedicalsDelivering the most effective grinding for all kind of sample in 40 seconds or less.