Evaluation of pH stability for an organosilica-based HPLC stationary phase

Silica-based stationary phases are the workhorse for most HPLC methods. Mechanical strength, availability, and options for chemically bonding the stationary phase are a few of the many reasons for this. However, one significant limitation for pure silica base particles is the operating pH range. Organosilanes have been developed to extend the working pH range for silica-based HPLC media and are well-suited to these high pH applications. In this application note from YMC America, Inc., explore a process to evaluate the pH stability of an organosilane HPLC media with an extended pH range and report the obtained results.