Application Note: Automation and Image-Based Analysis of a Hanging Drop MicroHole Plate Model to Create and Differentiate 3D Mesenchymal Stem Cell Spheroids for Downstream Tissue Formation

18 August 2017

Human mesenchymal stem cells (hMSCs) have emerged as a leading candidate in cellular therapies due to their unique properties. This application note demonstrates a method to automate the steps necessary to culture 3D hMSCs spheroids and monitor them during tissue repair and regeneration. BioTek’s Cytation™ 5 Cell Imaging Multi-Mode Reader was used to monitor spheroids in the inserts during the differentiation process using the brightfield channel, as well as to capture fluorescent images to verify protein marker expression and chondrocyte differentiation. 

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