Application Note: Automated Imaging-Based Technique for Monitoring of Ca2+ and DAG Biosensors Enables Detailed Characterization of GPCR Kinetics
8 November 2018

This poster describes an automated imaging-based approach to characterize the IP3 /DAG signaling pathway using multiplexed R-GECO Ca2+ and DAG fluorescent biosensors from Montana Molecular. This method produces detailed kinetic profiles of Ca2+ flux and DAG levels following activation of Gq-coupled receptors within the same population of cells. Background subtraction and image analysis tools enable detection of changes in Ca2+ and DAG levels that are more sensitive than techniques relying on bulk fluorescence measurements. Additionally, this method enables single-cell analysis for detailed characterization of response dynamics and subcellular effects.