Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate

Sample preparation protocols specify the need for “wide bore” pipette tips and cannulas to minimize the inadvertent harm, which can be applied to native biological sample matrices during sample preparation. Loss of biological sample integrity can occur due to the pipette tips’ small internal orifice diameter and the speed of aspiration and dispense during pipetting. Biomek Wide Bore Pipette Tips have been designed to address such sample preparation challenges. Through the use of Computational Fluid Dynamics (CFD) software, physical forces and flow characteristics within a pipette tip during pipetting have been simulated. Comparisons of pipette tip orifice diameters can be analyzed for such parameters as fluid (sample) velocity, wall shear, and shear strain rate.