ResourceLife Sciences
Validate your antibodies
11 Apr 2021When it comes to research use, there are no standard guidelines in place for manufacturing, validating, and using antibodies.
In this poster, Bethyl Laboratories recommend the methods and key questions to ask when purchasing antibodies to ensure their validity.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.