Separation and characterization of NISTmAb charge variants with CEInfinite on-line iCIEF-MS
15 Oct 2019On-line iCIEF-MS coupling using the CEInfinite Preparative iCIEF instrument system was verified by the congruent charge isomer peaks between the iCIEF separation of the NISTmAb and its input into the MS, as well as the successful identification of the individual peaks. This technique can easily be applied to other mAbs as well as other protein-based biopharmaceuticals with little additional development from the initial iCIEF method.
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CEInfinite
Isogen Life ScienceCEInfinite Preparative facilitates charge variant characterization by direct MS-detection and automated fractionation. CEInfinite is a whole column imaging CIEF, which allows fast method development and high throughput compared to traditional CE. The system is known for its robustness and is FDA CFR 21 Part 11 compliant