Scaling the potential of patient derived organoids through automation and label free image analysis of individual organoids

29 Mar 2023

3D patient-derived tumor organoids hold great potential for many basic and translational applications. However, traditional approaches to organoid image analysis and downstream data processing are often challenging and labor-intensive. Download this presentation to discover how Dr. Christophe Deben, Group Leader of the University of Antwerp’s Tumoroid Screening Lab, has developed a label-free brightfield image analysis software (OrBITS) to monitor organoid growth rates and track movements.

Dr. Deben also shares a semi-automated workflow for cell seeding drug treatment using the Tecan D300e digital drug dispenser and live-cell imaging using the Tecan Spark® Cyto multimode plate reader.

Spark® Cyto

Tecan

Spark Cyto is the first live cell plate reader to detect biological, chemical or physical events – capturing the maximum amount of data possible from each plate well, at the same time and under the same conditions.

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D300e Digital Dispenser

Tecan

The D300e Digital Dispenser* uses direct digital dispensing technology to enable rapid delivery of picoliter to microliter sample volumes. It delivers fast, reliable performance across a large dynamic range, eliminating the need for slow, error-prone and wasteful serial dilutions for creation of dose-response curves and enabling miniaturized qPCR reaction set-up.

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