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Preparation of bacterial samples for sequencing

17 Apr 2024

It is estimated that hospital-associated infections (HAI) cost between $35 and $45 billion in the USA and the NHS £3 billion in the UK, as well as countless lives. To counter such costs, Microzone proposes a method for the rapid preparation of cultured bacterial samples for Sanger Sequencing. Utilization of Microzone's 'free from' technology is highlighted for allowing for fast, efficient workflows only requiring a centrifugation step and not requiring automation.

MegaMix

Microzone

MegaMix: All-in-one PCR solution with Taq DNA polymerase, 220 μM dNTP, and 2.75 mM MgCl2 in Microzone’s enhancing buffer for swift, reliable, and high-yield amplification.

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microCLEAN

Microzone

microCLEAN is part of the Microzone Free From range of products. There are no filter columns, magnetic beads, alcohols nor solvents used.  microCLEAN removes unwanted residual dNTPs, short primer dimers, unincorporated dyes etc post PCR. Quick and simple protocol with minimal equipment required.

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