Application Note: Identifying metabolic phenotype switches in cancer cells in an hypoxic environment
7 June 2023

Decreased oxygen levels, or hypoxia, and hypoxic-mediated signaling play an important role in the pathology of diseases including stroke, ischemic heart disease, renal fibrosis, and cancer. In this application note, learn more about the metabolic differences in MCF-7 human breast cancer cells cultured and assayed at either ambient or 5 % oxygen levels, by measuring mitochondrial respiration and glycolytic activity using the Agilent Seahorse XF Analyzer and XF Hypoxia Rate Calculator.