Identifying metabolic phenotype switches in cancer cells in an hypoxic environment
6 Jun 2023Decreased oxygen levels, or hypoxia, and hypoxic-mediated signaling play an important role in the pathology of diseases including stroke, ischemic heart disease, renal fibrosis, and cancer. In this application note, learn more about the metabolic differences in MCF-7 human breast cancer cells cultured and assayed at either ambient or 5 % oxygen levels, by measuring mitochondrial respiration and glycolytic activity using the Agilent Seahorse XF Analyzer and XF Hypoxia Rate Calculator.
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Seahorse XF Pro Analyzer
Agilent TechnologiesMeasure mitochondrial respiration, glycolysis, and ATP production rates in live cells in a 96-well forma t, providing a system-level view of cellular energy metabolism and mitochondrial health. It combines enhanced sensitivity and data consistency with intuitive software and advanced data analytics, enabling users to streamline assay workflow from start to finish and generate and interpret metabolic data easily.