Application Note: How to calculate the molecular weight of nucleic acids
13 September 2023

For some applications, it is helpful to know the molecular weight of an oligonucleotide in order to calculate the amount of substance in a solution or to estimate the suitable particle pore size for analysis by size exclusion chromatography (SEC). In this application note, YMC Europe provides assistance in the calculation of the molecular weight of single-stranded as well as double-stranded DNA and RNA molecules. It also contains typical molecular weights of poly(dT) oligonucleotides and naturally occurring RNAs.