Application Note: Genomic DNA handling and the Agilent Femto Pulse system
6 June 2023
In this application note, Agilent Technologies discusses the significance of genomic DNA (gDNA) integrity in molecular biology labs. Maintaining high-quality samples is crucial to generating reliable results and saving time and resources. Various factors such as extraction techniques, storage conditions, and mixing can affect gDNA sample integrity. Pulsed field gel electrophoresis (PFGE) is commonly used for quality assessment but requires significant sample quantities and overnight runs. Agilent Femto Pulse system with Genomic DNA 165 kb kit is a faster and cost-effective alternative that offers high sensitivity and resolution. This note examines the impact of different handling protocols on gDNA sample integrity and provides recommendations for best practices when using the Femto Pulse.
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