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An improved workflow for profiling and quantitation of sialic acids in biotherapeutics

18 Nov 2021

This application note describes the use of the Agilent AdvanceBio Sialic Acid profiling and quantitation kit to profile and perform absolute quantitation of sialic acids present in biotherapeutic glycoproteins as well as the NISTmAb.

Agilent 1290 Infinity II LC System

Agilent Technologies

The 1290 Infinity II LC System is an ultra-high performance LC that has market-leading sample capacity and is designed for the lowest dispersion for the most challenging ultra-HPLC experiments. The 1290 Infinity II LC system is designed to be both a HPLC and high-end UHPLC system in a single flow path, giving you robust and excellent performance no matter what application you are running while seamlessly integrating into your existing laboratory environment.

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6545XT AdvanceBio LC/Q-TOF

Agilent Technologies

The Agilent 6545XT AdvanceBio LC/Q-TOF system is designed to handle multiple workflows in biopharmaceutical characterization. When you need to access the most detailed information at the intact protein level, automatically confirm protein sequence at the peptide level, and confidently understand post-translational modifications, the 6545XT AdvanceBio Q-TOF delivers results you can trust.

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An improved workflow for profiling and quantitation of sialic acids in biotherapeutics