Product & ReviewsAntibodies
RPE65 Antibody (401.8B11.3D9)
Product Details
- Cat. No.
- NB100-355
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
![Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ad9151bf265a37ca610fad5354a659dff4bef9dd-400x299.jpg?w=1080&q=75&fit=clip&auto=format)
Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.
![Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ad9151bf265a37ca610fad5354a659dff4bef9dd-400x299.jpg?w=256&q=75&fit=clip&auto=format)
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Description
RPE65 Antibody (401.8B11.3D9)
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Bovine, Human, Mouse, Pig (Swine)
- Antigen: RPE65
- Isotype: IgG1 Kappa
- Source: Protein G purified
Handling
- Quantity: 0.025 ml
- Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
- Buffer: PBS
Applications
- Flow Cytometry (FC)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Western Blotting (WB)
![Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ad9151bf265a37ca610fad5354a659dff4bef9dd-400x299.jpg?w=3840&q=75&fit=clip&auto=format)