Size exclusion chromatography of recombinant Human IgG4 Kappa
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Recombinant Human IgG4 kappa, AbD18705_hIgG4 is a recombinant human IgG4 antibody with a kappa light chain.AbD18705_hIgG4 is specific for green fluorescent protein (GFP) and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG4 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.In addition to the tetrameric IgG structure, composed of two copies each of the heavy and light chains, human IgG4 forms so-called half-molecules. This species contains only one heavy and one light chain. Half-molecules can assemble with an unrelated half-molecule to form bispecific tetrameric antibody species (Aalberse et al. 2002). Half-molecules of human IgG4 preparations can be detected as an additional species under denaturing, non-reducing conditions using SDS-PAGE, size exclusion chromatography or capillary electrophoresis.Mutation in the core hinge region of IgG4 prevents the formation of half-molecules (Bloom et al. 1997). In addition to the wild type recombinant, Bio-Rad also offers recombinant Human IgG4 kappa in its mutated form (HCA247). Recombinant Human IgG4 lambda is also available in both wild type (HCA050) and mutant form (HCA246).
