PLK1 [p Thr210] Antibody
Product Details
- Cat. No.
- NBP1-78021
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/15c8f3552784a79934ca638522680f837e02063d-400x49.jpg?w=1080&q=75&fit=clip&auto=format)
Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/15c8f3552784a79934ca638522680f837e02063d-400x49.jpg?w=256&q=75&fit=clip&auto=format)
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Description
PLK1 [p Thr210] Antibody
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Human
- Antigen: PLK1
- Modifications: p Thr210
- Source: Immunogen affinity purified
Handling
- Quantity: 0.1 mg
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Applications
- ELISA (ELISA)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Western Blotting (WB)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/15c8f3552784a79934ca638522680f837e02063d-400x49.jpg?w=3840&q=75&fit=clip&auto=format)