Product & ReviewsAntibodies
Perforin Antibody (CB5.4)
Product Details
- Cat. No.
- NBP1-97512
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Rat
![Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species. Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/eb28ae1a75058b90fa087138adf71f37d28b7b63-400x383.jpg?w=1080&q=75&fit=clip&auto=format)
Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species. Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
![Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species. Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/eb28ae1a75058b90fa087138adf71f37d28b7b63-400x383.jpg?w=256&q=75&fit=clip&auto=format)
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Description
Perforin Antibody (CB5.4)
Biological Information
- Clonality: Monoclonal
- Host: Rat
- Reactivity: Mouse
- Antigen: Perforin
- Isotype: IgG2a
- Source: Protein G purified
Handling
- Quantity: 0.1 mg
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: Liquid. In PBS
Applications
- Flow Cytometry (FC)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Immunoprecipitation (IP)
- Western Blotting (WB)
![Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species. Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/eb28ae1a75058b90fa087138adf71f37d28b7b63-400x383.jpg?w=3840&q=75&fit=clip&auto=format)