Product & ReviewsAntibodies
p53 Antibody (PAb 1801)
Product Details
- Cat. No.
- NB200-104
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
![Immunoprecipitation: p53 Antibody (PAb 1801) [NB200-104] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by NB200-104 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit was used in Western blot.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/829ac79cf3c0f94968b6d1c0a6051b59147b6b55-293x400.jpg?w=1080&q=75&fit=clip&auto=format)
Immunoprecipitation: p53 Antibody (PAb 1801) [NB200-104] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by NB200-104 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit was used in Western blot.
![Immunoprecipitation: p53 Antibody (PAb 1801) [NB200-104] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by NB200-104 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit was used in Western blot.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/829ac79cf3c0f94968b6d1c0a6051b59147b6b55-293x400.jpg?w=256&q=75&fit=clip&auto=format)
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Description
p53 Antibody (PAb 1801)
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Human
- Antigen: p53
- Isotype: IgG1
- Source: Protein G purified
Handling
- Quantity: 100 ul
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: PBS, pH 7.4
Applications
- Chromatin Immunoprecipitation (ChIP)
- ELISA (ELISA)
- Flow Cytometry (FC)
- Immunocytochemistry (ICC)
- Immunohistochemistry (Frozen Sections) (IHC (Fr))
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Immunoprecipitation (IP)
- Radioimmunoassay (RIA)
- Western Blotting (WB)
![Immunoprecipitation: p53 Antibody (PAb 1801) [NB200-104] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by NB200-104 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit was used in Western blot.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/829ac79cf3c0f94968b6d1c0a6051b59147b6b55-293x400.jpg?w=3840&q=75&fit=clip&auto=format)