Western blot analysis of HepG2 human hepatic carcinoma whole cell lysate
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Mouse anti Human GAPDH antibody, clone 4G5 recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 36 kDa protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death (Chuang & Ishitani 1996). GAPDH binds to several proteins responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Burke et al. 1996).
The antibody was great.
Western blot
I was using the antibody for the purpose of western blot. I used 1:3000 dilution and it was good.
Review Date: 2 Oct 2019 | Bio-Rad
