mCherry Antibody
Product Details
- Cat. No.
- NBP2-25157
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
![Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with NBP2-25157 is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8cd678e2d810b3efa30d571323ec12f2ff708ee1-400x400.jpg?w=1080&q=75&fit=clip&auto=format)
Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with NBP2-25157 is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
![Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with NBP2-25157 is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8cd678e2d810b3efa30d571323ec12f2ff708ee1-400x400.jpg?w=256&q=75&fit=clip&auto=format)
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Description
mCherry Antibody
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Not Species Specific
- Antigen: mCherry
- Source: Immunogen affinity purified
Handling
- Quantity: 0.1 ml
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: PBS
Applications
- Immunocytochemistry (ICC)
- Western Blotting (WB)
![Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with NBP2-25157 is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8cd678e2d810b3efa30d571323ec12f2ff708ee1-400x400.jpg?w=3840&q=75&fit=clip&auto=format)