Product & ReviewsAntibodies
LC3B/MAP1LC3B Antibody (1251A)
Product Details
- Cat. No.
- NBP2-46892
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Rabbit
![Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] - HeLa cells were treated with 50 uM Chloroquine for 24 hour prior to fixation in 10% buffered formalin for 10 min. Cells were permeabilized in 0.1% Triton X-100 and incubated with 20 ug/ml anti-LC3B (1251A) and 1:500 anti-tubulin NB100-690) for 1 h at room temperature. LC3 reactivity (green) was detected with ant-rabbit Dylight 488 and tubulin (red) with anti-mouse Dylight 550. Nuclei were counterstained with DAPI (blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/2b3e119510269f052afb51b5795d3e65dc3f328d-400x303.jpg?w=1080&q=75&fit=clip&auto=format)
Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] - HeLa cells were treated with 50 uM Chloroquine for 24 hour prior to fixation in 10% buffered formalin for 10 min. Cells were permeabilized in 0.1% Triton X-100 and incubated with 20 ug/ml anti-LC3B (1251A) and 1:500 anti-tubulin NB100-690) for 1 h at room temperature. LC3 reactivity (green) was detected with ant-rabbit Dylight 488 and tubulin (red) with anti-mouse Dylight 550. Nuclei were counterstained with DAPI (blue).
![Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] - HeLa cells were treated with 50 uM Chloroquine for 24 hour prior to fixation in 10% buffered formalin for 10 min. Cells were permeabilized in 0.1% Triton X-100 and incubated with 20 ug/ml anti-LC3B (1251A) and 1:500 anti-tubulin NB100-690) for 1 h at room temperature. LC3 reactivity (green) was detected with ant-rabbit Dylight 488 and tubulin (red) with anti-mouse Dylight 550. Nuclei were counterstained with DAPI (blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/2b3e119510269f052afb51b5795d3e65dc3f328d-400x303.jpg?w=256&q=75&fit=clip&auto=format)
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Description
LC3B/MAP1LC3B Antibody (1251A)
Biological Information
- Clonality: Monoclonal
- Host: Rabbit
- Reactivity: Human, Mouse
- Antigen: LC3B/MAP1LC3B
- Isotype: IgG
- Source: Protein G purified
Handling
- Quantity: 0.025 mg
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: PBS
Applications
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Western Blotting (WB)
![Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] - HeLa cells were treated with 50 uM Chloroquine for 24 hour prior to fixation in 10% buffered formalin for 10 min. Cells were permeabilized in 0.1% Triton X-100 and incubated with 20 ug/ml anti-LC3B (1251A) and 1:500 anti-tubulin NB100-690) for 1 h at room temperature. LC3 reactivity (green) was detected with ant-rabbit Dylight 488 and tubulin (red) with anti-mouse Dylight 550. Nuclei were counterstained with DAPI (blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/2b3e119510269f052afb51b5795d3e65dc3f328d-400x303.jpg?w=3840&q=75&fit=clip&auto=format)