Product & ReviewsAntibodies
HIF-1 alpha Antibody
Product Details
- Cat. No.
- NB100-449
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
![Flow Cytometry: HIF-1 alpha Antibody [NB100-449] - Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.125ug anti- HIF-alpha and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Red- untreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/048f83b9ca705f1fe8583ea09f7991a6dc986ae4-370x400.jpg?w=1080&q=75&fit=clip&auto=format)
Flow Cytometry: HIF-1 alpha Antibody [NB100-449] - Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.125ug anti- HIF-alpha and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Red- untreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.
![Flow Cytometry: HIF-1 alpha Antibody [NB100-449] - Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.125ug anti- HIF-alpha and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Red- untreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/048f83b9ca705f1fe8583ea09f7991a6dc986ae4-370x400.jpg?w=256&q=75&fit=clip&auto=format)
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Description
HIF-1 alpha Antibody
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Chicken, Human, Monkey, Mouse
- Antigen: HIF-1 alpha
- Source: Immunogen affinity purified
Handling
- Quantity: 0.1 ml
- Storage: Store at 4C. Do not freeze.
- Buffer: Tris-buffered Saline and 0.1% BSA
Applications
- Chromatin Immunoprecipitation (ChIP)
- ELISA (ELISA)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunohistochemistry (Frozen Sections) (IHC (Fr))
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Immunoprecipitation (IP)
- Western Blotting (WB)
![Flow Cytometry: HIF-1 alpha Antibody [NB100-449] - Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.125ug anti- HIF-alpha and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Red- untreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/048f83b9ca705f1fe8583ea09f7991a6dc986ae4-370x400.jpg?w=3840&q=75&fit=clip&auto=format)