GLUT10 Antibody
Product Details
- Cat. No.
- NB100-490
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
![Immunocytochemistry/Immunofluorescence: GLUT10 Antibody [NB100-490] - Dissected inner ear tissues from mice immersed in 20% sucrose PBS solution (pH7.4) followed by being embedded in OCT. Tissues snap frozen in liquid nitrogen & 7um sections were cut by cryostat. Cochlear sections from organ of Corti were dissected & rinsed by 0.1% Triton in PBS (pH7.4,PBST) for 30min. Sections were blocked with 5% goat serum in PBST at RT 1hr. NB100-490 was used at 1:200 to label the cochlear sections at 4C. Sections were incubated with secondary (anti-rabbit Cy3, 1:400) RT 1 hr. Slides mounted with fluoromont G and examined under a confocal microscope. Strong immunoreactivity (red) is observed in the inner and outer hair cells and basilar membrane. Spiral ganglion neurons display moderate staining.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/d942a2f5af80a9a109ea87efa03825872b462b2e-400x315.jpg?w=1080&q=75&fit=clip&auto=format)
Immunocytochemistry/Immunofluorescence: GLUT10 Antibody [NB100-490] - Dissected inner ear tissues from mice immersed in 20% sucrose PBS solution (pH7.4) followed by being embedded in OCT. Tissues snap frozen in liquid nitrogen & 7um sections were cut by cryostat. Cochlear sections from organ of Corti were dissected & rinsed by 0.1% Triton in PBS (pH7.4,PBST) for 30min. Sections were blocked with 5% goat serum in PBST at RT 1hr. NB100-490 was used at 1:200 to label the cochlear sections at 4C. Sections were incubated with secondary (anti-rabbit Cy3, 1:400) RT 1 hr. Slides mounted with fluoromont G and examined under a confocal microscope. Strong immunoreactivity (red) is observed in the inner and outer hair cells and basilar membrane. Spiral ganglion neurons display moderate staining.
![Immunocytochemistry/Immunofluorescence: GLUT10 Antibody [NB100-490] - Dissected inner ear tissues from mice immersed in 20% sucrose PBS solution (pH7.4) followed by being embedded in OCT. Tissues snap frozen in liquid nitrogen & 7um sections were cut by cryostat. Cochlear sections from organ of Corti were dissected & rinsed by 0.1% Triton in PBS (pH7.4,PBST) for 30min. Sections were blocked with 5% goat serum in PBST at RT 1hr. NB100-490 was used at 1:200 to label the cochlear sections at 4C. Sections were incubated with secondary (anti-rabbit Cy3, 1:400) RT 1 hr. Slides mounted with fluoromont G and examined under a confocal microscope. Strong immunoreactivity (red) is observed in the inner and outer hair cells and basilar membrane. Spiral ganglion neurons display moderate staining.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/d942a2f5af80a9a109ea87efa03825872b462b2e-400x315.jpg?w=256&q=75&fit=clip&auto=format)
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Description
GLUT10 Antibody
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Human, Mouse
- Antigen: GLUT10
- Source: Immunogen affinity purified
Handling
- Quantity: 0.1 mg
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: PBS
Applications
- Immunocytochemistry (ICC)
- Western Blotting (WB)
![Immunocytochemistry/Immunofluorescence: GLUT10 Antibody [NB100-490] - Dissected inner ear tissues from mice immersed in 20% sucrose PBS solution (pH7.4) followed by being embedded in OCT. Tissues snap frozen in liquid nitrogen & 7um sections were cut by cryostat. Cochlear sections from organ of Corti were dissected & rinsed by 0.1% Triton in PBS (pH7.4,PBST) for 30min. Sections were blocked with 5% goat serum in PBST at RT 1hr. NB100-490 was used at 1:200 to label the cochlear sections at 4C. Sections were incubated with secondary (anti-rabbit Cy3, 1:400) RT 1 hr. Slides mounted with fluoromont G and examined under a confocal microscope. Strong immunoreactivity (red) is observed in the inner and outer hair cells and basilar membrane. Spiral ganglion neurons display moderate staining.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/d942a2f5af80a9a109ea87efa03825872b462b2e-400x315.jpg?w=3840&q=75&fit=clip&auto=format)