Product & ReviewsAntibodies
E6AP/UBE3A Antibody
Product Details
- Cat. No.
- NB500-240
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
![Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6ee5ccbe70cf331edc8fc6b6ac556ad46b561e5b-400x264.jpg?w=1080&q=75&fit=clip&auto=format)
Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
![Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6ee5ccbe70cf331edc8fc6b6ac556ad46b561e5b-400x264.jpg?w=256&q=75&fit=clip&auto=format)
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Description
E6AP/UBE3A Antibody
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Human, Mouse
- Antigen: E6AP/UBE3A
- Source: Immunogen affinity purified
Handling
- Quantity: 100 ul
- Storage: Store at 4C. Do not freeze.
- Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
Applications
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blotting (WB)
![Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6ee5ccbe70cf331edc8fc6b6ac556ad46b561e5b-400x264.jpg?w=3840&q=75&fit=clip&auto=format)