Western Blot. Positive WB detected in: HepG2 whole cell lysate, Rat Heart whole cell lysate, Rat Brain whole cell lysate. All lanes: CYP17A1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 kDa. Observed band size: 58 kDa.