Western BlotPositive WB detected in:A431 whole cell lysate (20µg), SH-SY5Y whole cell lysate (20µg), U251 whole cell lysate (20µg), 293T whole cell lysate (20µg), HepG2 whole cell lysate (20µg), K562 whole cell lysate (20µg), MCF7 whole cell lysate (20µg), NIH/3T3 whole cell lysate (20µg)All lanes: TUBB antibody at 1:1000SecondaryGoat polyclonal to human IgG at 1/40000 dilutionPredicted band size:49 kDaObserved band size:55 kDaExposure time:5s