Western Blot. Positive WB detected in 293 whole cell lysate,A549 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF). All lanes Phospho-ATF2 antibody at 1.015μg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70 KDa. Observed band size: 70 KDa.