Western BlotPositive WB detected in:U251 whole cell lysate (20µg), Hela whole cell lysate (20µg), HepG2 whole cell lysate (20µg), 293T whole cell lysate (20µg), A549 whole cell lysate (20µg), MCF7 whole cell lysate (20µg)All lanes: ACTB antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/40000 dilutionPredicted band size:42 kDaObserved band size:42 kDaExposure time:20s