Western blot. All lanes: MPP6 antibody at 3.58ug/ml. Lane 1: Mouse brain tissue. Lane 2: Mouse kidney tissue. Lane 3: HepG2 whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 61 kDa. Observed band size: 61 kDa.