Western blot. All lanes: NUP58 antibody at 4ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: LO2 whole cell lysate. Lane 4: Mouse thymus tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 61, 51, 60 kDa. Observed band size: 61 kDa.