Western blot. All lanes: RANBP3 antibody at 6.4ug/ml. Lane 1: Mouse brain tissue. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 61, 60, 54 kDa. Observed band size: 61 kDa.