Western Blot. Positive WB detected in: HeLa whole cell lysate, HL-60 whole cell lysate, K562 whole cell lysate, 293T whole cell lysate. All lanes: DDX47 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 46 kDa. Observed band size: 51 kDa.