Western blot. All lanes: PIP5K1A antibody at 4.12ug/ml. Lane 1: Hela whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: Mouse heart tissue. Lane 4: Mouse kidney tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 63, 57, 62, 59 kDa. Observed band size: 63 kDa.