Western blot. All lanes: RNF5 antibody at 3.08µg/ml. Lane 1: A431 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 20 kDa. Observed band size: 20 kDa