Western blot. All lanes: TMBIM1 antibody at 8µg/ml. Lane 1: U251 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: Mouse lung tissue. Lane 6: Mouse liver tissue. Lane 7: Mouse kidney tissue. Lane 8: Human placenta tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 35 kDa. Observed band size: 35 kDa