Western blot. All lanes: WDYHV1 antibody at 6ug/ml. Lane 1: Mouse heart tissue. Lane 2: MCF-7 whole cell lysate. Lane 3: U251 whole cell lysate. Secondary. Goat polyclonal to Rabbit IgG at 1/10000 dilution. Predicted band size: 24, 17 kDa. Observed band size: 24 kDa.