Western blot. All lanes: Twinfilin-2 antibody at 3ug/ml. Lane 1: Jurkat whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 40 kDa. Observed band size: 40 kDa.